Michael Bailes wrote: "[The Scoville] test was a dilution type test which used a panel of five taste testers to arrive at a rating, which was measured in increments of 100 Scoville units. Controlled amounts of Chilli were given to tasters. Tasters then recorded pungency in multiples of one hundred. A majority of three tasters had to agree before a value was assigned to the food." A nitpick, but an important one. The language quoted implied the tasters would themselves assign a heat value based on their individual assessment. My impression is that this is not correct. The method of the Scoville test is a threshold one. The procedure was to dilute the chile powder or preparation, or whatever (remember, Wilber S. was testing content for medicinal, not culinary, application) until it was just detectable. "Detectable" meant that 3 of the 5 panelists said they detected pepper heat in the preparation at a threshold level. The number of Scoville Units assigned was the inverse of the dilution to the nearest 100 units. For instance, suppose you extracted 1 gram of powder and made a "stock" by diluting it to 100 ml. If this was detected by the panel, you might dilute the "stock" preparation 1 in 2, 1 in 4 and 1 in 8. Supposing the scoring went like so: Preparation Panelists Detecting heat 1 in 2 all 1 in 4 3 of 5 1 in 8 1 of 5 The dilution required for threshold was (1/100) x (1/2) or 1/200. The original powder would then be assigned 200 Scoville units. Sharp eyes might detect the uncertainty introduced by not having tried 1 in 5, 1 in 6 or 1 in 7. A good application of the test would include these, and illustrates how applying the procedure might become tedious if heat levels were not known fairly well beforehand. However, there are a number of important implications of this methodology as opposed to the one implied in the quote. First and foremost, it simply uses the sense of taste as a detector and works at the threshold. The question asked about each sample is "Can you detect anything?" rather than "How hot do you think this is?" It is therefore "digital" (yes or no) rather than "analog" (around xx## units). The trick is to make the right dilutions to present to the panel. Panelists can be minimally trained, or receive no training at all. Finally, they do not extinguish as rapidly because their exposure is always to very low levels of capsaicinoids. It is not a "torture test" for the panelists (not very much fun for a Chile-Head). It is time-consuming and tricky since panelists should "rest" after two or at most three successful detections. To be efficient, you have to get the dilutions nearly correct each time. ASTM 1083 is a "shortcut" that does ask the panelists to rate the heat by placing a mark on a graduated scale after doing a taste comparison with a known dilution of an N-vanillyl-n-nonamide standard. Since exposure levels are higher, no more than two determinations are to be made in one day for this one. ASTM claims good correlation with HPLC (r = 0.94). Again, Chile-Heads need not apply as tasters. George Nelson 70431.3065@compuserve.com