Awhile ago there was a short discussion about ways to measure capsaicin. Besides the two ways mentioned (HPLC and using dilutions with human tasters), there seems to be a new way. I've pasted some info from the website below. The URL for the company is: www.neogen.com I have not tried this method. By the way, I saw "Eat the Heat" and thought it was great! --Mark Neogen releases rapid test to measure capsaicin FOR IMMEDIATE RELEASE CONTACTS: Chuck Bird, Neogen Technical Services Manager Tim Hendra, Neogen Diagnostic Sales Director 517/372-9200 LANSING, Mich., Nov. 14, 2000 - Neogen Corporation's (Nasdaq: NEOG) new quantitative test for capsaicin provides testers with a quicker, less expensive alternative to methods currently used. Capsaicin is the compound that gives peppers their hotness. The compound is used in a wide range of concentrations in the production of spicy food products, such as salsa, sauces, seasonings and mixes. More than 300 companies in the United States alone use capsaicin in their products, and many of them test for capsaicin levels to ensure product consistency and labeling accuracy. "Companies that currently test for capsaicin generally run their own high performance liquid chromatography (HPLC) or gas chromatography units, or send samples to an outside lab for testing," said Tim Hendra, Neogen's diagnostic sales director. "The current methods yield accurate, but relatively slow and expensive results. The start-up costs for HPLC testing, for example, are in the $50,000 range, and are beyond the reach of many smaller companies. And, HPLC allows only the testing of a single sample at a time." "Neogen's new test, Veratox® for Capsaicin, requires only minor start-up costs, and allows for the batching of up to 20 samples at a time - a big time saver that allows companies to move their products much more quickly through the production cycle," Hendra continued. "This test is an excellent alternative for smaller companies who could perform simple in-house testing instead of sending their samples out, and large companies that could benefit from a much quicker testing turnaround." Neogen's new test is a competitive direct enzyme-linked immunosorbent assay (ELISA) that allows the testing of up to 20 samples in about 20 minutes. Outside laboratories take 1 or 2 days to deliver results, at about $75 a test. Veratox for Capsaicin delivers results at about $8 per test. Neogen Corporation develops and markets products and services dedicated to food and animal safety. The Company's Food Safety Division markets diagnostic test kits to detect foodborne bacteria, natural toxins, genetic modifications, food allergens, drug residues, plant diseases and sanitation concerns. Neogen's Animal Safety Division markets a complete line of diagnostics, veterinary instruments, veterinary pharmaceuticals, nutritional supplements and wound care products.